Parker accidentally activated a new function in his Web-Shooters while checking them when his aunt May Parker entered into his room to take care of his blackened eye, forcing Parker to hide the device behind a cushion. Once she left, he noticed a red light beam being projected from the Web-Shooter and looked at the roof to notice a signal very similar to the mask of his new costume. Parker watched the signal in delighted surprise.
After tagging Shocker with a Spider-Tracer, Parker and best friend Ned Leeds returned to the former's room to track down Adrian Toomes' Crew, during which Parker emits the Spider-Signal on his bedroom wall.
The Spider-Signal is a red light emitted from Spider-Man's upgraded web-shooters, projecting a signal very similar to the mask of his profound suit. Peter Parker has operated this gimmick a few times in his activities as a superhero, such as using it to project light in his bedroom or checking his web-shooter functions.
- In the comics, the Spider-Signal is usually emitted from Spider-Man's belt instead of his Web-Shooters.