- "You shouldn't have come down here."
- ―John Bruno to Bobbi Morse
John Bruno is a former military assassin who was put into S.H.I.E.L.D. custody for discovering creative ways to kill.
Bruno was imprisoned in the sub-basement of the Brynmore Psychiatric Facility in Ohio along with David Angar. When Calvin Zabo broke into the prison to recruit Angar, he had Wendell Levi shut down the power, freeing the prisoners. Bruno waited in the sub-basement until Phil Coulson and Bobbi Morse came to investigate what happened at the facility. Bruno attacked Morse, but she easily overpowered him.
- Combatant: As a former military assassin, Bruno is a skilled fighter, using creative ways to kill. However, he was not able to hold his own against Bobbi Morse for long.