Agent Jacobson was a S.H.I.E.L.D. operative assigned to the Hub. During the HYDRA Uprising he worked with Victoria Hand to maintain the security of the Hub against HYDRA. While transporting the HYDRA infiltrator, John Garrett also known as the Clairvoyant, Jacobson was murdered by Grant Ward.
When the HYDRA infiltration of S.H.I.E.L.D. was discovered, Jacobson was one of five other agents that Victoria Hand knew was not a sleeper agent. Jacobson was in the Hub when Hand gave the order to kill Coulson's Team and Agent John Garrett since she believed they were traitors. When Hand wanted to prove to Jemma Simmons that Phil Coulson was a HYDRA agent, she asked Jacobson to list Coulson's "crimes" against S.H.I.E.L.D. He gave a list of the various exploits that Coulson's team had, including not reporting on Akela Amador.
After Coulson's team arrived and Garrett's treason was revealed, Garrett was to be transported to the Fridge by Jacobson, Chaimson, Hand and Grant Ward. En route, Hand asked Ward to kill Garrett, so as to eliminate his threat. Instead, Ward shot and killed Hand, Chaimson and Jacobson.
- S.H.I.E.L.D. (First Incarnation)