The Arctic HYDRA Research Facility was one of HYDRA's main research facilities, specifically designed to research on enhanced people. The facility was commanded by List, the right hand man of Baron Strucker. Once Ethan Johnston was captured by HYDRA, he was brought to the facility for experimentation. However, Johnston later died in the experiment's thirty seventh hour. List was frustrated with the experiments, as they had not discovered any quantum entanglements in Johnston's DNA that would allow him to understand Inhumans, as he ordered Paula to remove Johnston's corpse.
Having captured the pair of them during a clash at Calvin Zabo's Building, HYDRA used this facility to study Deathlok and Lincoln Campbell. Once the prisoners had woken up, they spoke to each other through the walls and discussed their situation, with Deathlok confessing that his Forearm Rocket Launcher had been disabled, so he could not break them out. The pair were then knocked unconscious and subjected to all Doctor List's experimentations, where they removed Deathlok's Backscatter X-Ray Eye Implant and Cybertek Prosthetic Leg, while also cutting open Campbell's chest to examine his DNA.
As List took a break from his experiments on Deathlok and Campbell, he rejoined Sunil Bakshi, who had been watching all of the operations, as he noted that it was unlikely that Campbell would survive the next round of experiments. List had then expressed his frustration over this, noting that only Wanda and Pietro Maximoff had been able to survive these experiments. However, they were interrupted by a HYDRA guard, who informed List and Bakshi that the Bus had been approaching the facility, as List ordered his soldiers to shoot down the S.H.I.E.L.D. plane.
Agent Phil Coulson and his team infiltrated the base by sacrificing the Bus to HYDRA's missile defense system while cloaking their Quinjet. The team later proceeded to rescue Mike Peterson and Lincoln Campbell from HYDRA and disable HYDRA's anti-aircraft missile defenses, allowing the S.H.I.E.L.D. jets to destroy the base.
While Coulson's Team were fulfilling their orders, Coulson secretly extracted intelligence regarding Loki's Scepter. Coulson later reported his findings to Maria Hill; she then inquired if Theta Protocol was ready.