No way

I dont think she has a reason to, and I dont think that she wants to join too

the SHIELD Natasha worked for kind of was dismantled at the end of 2nd Captain America. from my understanding, none of the Avengers joined any of the newer versions of SHIELD.

If they addressed AoS, Mack would for sure take Yelena in.

Well the last time she saw her family as a child was when she was being chased by Shield. And then she got turned into a child assassin. So I don’t think so

Yeah, on her end, it would be a tough sell. Though Coulson's team literally made a hacker set to take them all down into one of their most loyal members so...

But she didn’t have any prior hatred towards shield besides the redacted files with her parents which is the reason she joined

about the chase when Yelena was younger; from what I recall, Yelena was never told who. and I take she never found out who.

also, I do not recall any opinion from her about SHIELD; nor SHIELD being brought up in any of her scenes (movie or show).

There was a shield logo on the truck