Mine is when he's filming the events of Civil War and geeking out about all the heroes and wondering who the "new guy" is.
My favorite moment in a Spider-man movie, however, doesn't involve Peter. It's Ned and his epic "Um, watching porn?" moment.
I'm hoping that I'll get a new favorite Spider-man moment and it involves a certain blind lawyer-by-day, vigilante-by-night but time will tell....