NASA

"Dr. Selvig read an energy surge from the Tesseract four hours ago." "NASA didn't authorize Selvig to go to test phase."

- Phil Coulson and Nick Fury

NASA (National Aeronatics and Space Administration) is the agency of the United States government responsible for the country's civilian Space program, aerospace, and aeronautics research.

Monolith Mission
"NASA was always curious about the Monolith."

- Will Daniels

In 2001, NASA sent an astronaut, Will Daniels, and three scientists, Taylor, Brubaker, and Austin, into the Monolith to survey the planet on the other side of the portal until a rescue team could come to retrieve them.

Project Valhalla
Using the alias Jane Thompson, Rosalind Price worked for NASA on Project Valhalla, a short-lived initiative designed to detect useful or hazardous alien assets before they entered Earth's atmosphere.

Project P.E.G.A.S.U.S.
NASA worked with S.H.I.E.L.D. at the Joint Dark Energy Mission Facility in the Mojave Desert to research the Tesseract, along with Erik Selvig, to attempt to create an object with unlimited self-sustaining energy. When Loki arrived and began the Destruction of Project P.E.G.A.S.U.S., a NASA Scientist hid from the chaos.

Appearances
In chronological order:
 * Agents of S.H.I.E.L.D.
 * Season Three
 * 4,722 Hours (flashbacks)
 * The Avengers
 * Guardians of the Galaxy (logo)
 * Agents of S.H.I.E.L.D.
 * Season Three
 * 4,722 Hours (flashbacks)
 * Laws of Nature (mentioned)
 * 4,722 Hours (flashbacks)

Trivia

 * Peter Quill had a patch with the NASA logo on his backpack.